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Obstetrics and Gynecology International
Volume 2010, Article ID 437528, 4 pages
Clinical Study

Effects of Assisted Reproduction Technology on Placental Imprinted Gene Expression

Reproduction Center, Department of Obstetrics and Gynecology, Toho University Omori Medical Center, 6-11-1 Omori-Nishi, Ota-ku, Tokyo 143-8541, Japan

Received 30 September 2009; Accepted 16 June 2010

Academic Editor: Fan Jin

Copyright © 2010 Yukiko Katagiri et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We used placental tissue to compare the imprinted gene expression of IGF2, H19, KCNQ1OT1, and CDKN1C of singletons conceived via assisted reproduction technology (ART) with that of spontaneously conceived (SC) singletons. Of 989 singletons examined (ART 𝑛 = 6 5 ; SC 𝑛 = 9 2 4 ), neonatal weight was significantly lower ( 𝑃 < . 0 0 1 ) in the ART group than in the SC group, but placental weight showed no significant difference. Gene expression analyzed by real-time PCR was similar for both groups with appropriate-for-date (AFD) birth weight. H19 expression was suppressed in fetal growth retardation (FGR) cases in the ART and SC groups compared with AFD cases ( 𝑃 < . 0 2 and 𝑃 < . 0 5 , resp.). In contrast, CDKN1C expression was suppressed in FGR cases in the ART group ( 𝑃 < . 0 1 ) , while KCNQ1OT1 expression was hyperexpressed in FGR cases in the SC group ( 𝑃 < . 0 5 ) . As imprinted gene expression patterns differed between the ART and SC groups, we speculate that ART modifies epigenetic status even though the possibilities always exist.