Blocking Epidermal Growth Factor Receptor Signaling in HTR-8/SVneo First Trimester Trophoblast Cells Results in Dephosphorylation of PKB/AKT and Induces Apoptosis
Figure 1
(a) Kinexus multilane immunoblot showing untreated HTR-8/SVneo cells (control). (b) Kinexus multilane immunoblot showing HTR-8/SVneo cells treated for 5 min-with EGF (30 ng/mL). Thirty-one phosphorylated proteins were tracked. Twenty-four proteins (see Table 1) were phosphorylated in HTR-8/SV neo cells. Seven of the proteins were not phosphorylated. PKB/AKT is a principal target which is phosphorylated by EGF (see immunoblot band marked 16). (c) Kinexus multilane immunoblot showing HTR-8/SVneo cells treated for 20 h with Iressa (1 M) plus EGF (30 ng/mL) for 5 min. Phosphorylation of PKBα/AKT was downregulated in the presence of Iressa (immunoblot band marked by 16). (d) Kinexus multilane immunoblot showing HTR-8/SVneo cells treated for 20 h with Iressa (1 μM). Phosphorylation of PKBα/AKT is significantly downregulated (see band marked by 16). (e) This graph represents the fold induction or downregulation of pKBα/AKT in response to EGF, Iressa, EGF + Iressa normalized to baseline untreated cells.
