Obesity and the Endometrium: Adipocyte-Secreted Proinflammatory TNFα Cytokine Enhances the Proliferation of Human Endometrial Glandular Cells

Figure 5

Effects of anti-TNFα neutralizing antibodies on EGE cell proliferation. Conditioned media collected from SW872 cells grown to more than 80 percent confluence were centrifuged, filtered, and diluted to 1%. These conditioned media were treated with 1 ng/mL of anti-TNFα antibody for an hour at 37°C. The neutralized conditioned media were then added to EGE cells and cell proliferation measured using CyQuant assay. Results shown represent three separate experiments with comparable results. *𝑃<0.05 (mean ± SE; 𝑛=3).