Research Article

G Protein-Coupled Estrogen Receptor-Selective Ligands Modulate Endometrial Tumor Growth

Figure 1

GPER expression in type I and type II endometrial cancer cells. (a) Representative immunofluorescence images of Ishikawa H cells (type I) and Hec50 cells (type II) transfected with either control siRNA or siRNA targeting GPER. GPER is shown in green; nuclei are stained blue with DAPI. (b) Western blot of GPER and actin in untransfected, control siRNA- and GPER-targeted siRNA-transfected H cells and Hec50 cells. (c) Western blot quantitation of GPER expression relative to actin and normalized to untransfected Hec50 cells. Data represent mean s.e.m. from three experiments. compared to control siRNA.
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