Protein Kinase Cα Modulates Estrogen-Receptor-Dependent Transcription and Proliferation in Endometrial Cancer Cells

Figure 3

Estrogen and PKCα responses are ER dependent. HEC-50 cells, lacking ER, were transiently transfected with 0.5 μg pEREluc or 0.5 μg pPS2luc and 0.3 μg pCMVβ in the presence or absence of 0.5 μg pmyrPKCα or vector control (pCDNA3). Cells were treated with ±100 nM estradiol (E2), as indicated. Luciferase activity was normalized to β-galactosidase and promoter activity expressed as Relative Light Units (RLU). Data are mean ± s.e.m of 4 experiments conducted in triplicate.