4-Hydroxy-2-Nonenal-Modified Glyceraldehyde-3-Phosphate Dehydrogenase Is Degraded by Cathepsin G in Rat Neutrophils

Figure 2

Time course of HNE-modified GAPDH degradation by incubation with cell extracts from neutrophils. The cell extracts from neutrophils were incubated with eGAPDH and 100 μM HNE (empty circle) or with eGAPDH alone without HNE as a control (filled circle) at 37°C for the indicated times. Reaction products were separated on 12% SDS-PAGE gels and analyzed by Western blotting using an anti-GAPDH mAb. Data are mean ± SE (bars) values from four independent experiments. Significant decreases in GAPDH level compared to control are indicated by asterisks: *𝑃<0.05 and ***𝑃<0.001.