Chopper Is Prodeath Regardless of the Effect of p75ICD on Sensitivity to Oxidative Stress
Figure 2
(a) Results of transfection of HN33.11 cells with green fluorescent protein- (GFP-) linked empty plasmid (C2eGFP) or GFP-linked multiple cloning site in the same plasmid with a doxycycline- (Dox-) inducible internal ribosome entry site (MCSiresGFP). Dox elicits production of GFP in the MCSiresGFP-transfected cells, but not in C2eGFP-transfected cells. (b) Dox-inducibility of MCSiresGFP-linked expression constructs for (A) and (B) as defined in Figure 1. Open bars: cells maintained in the absence of Dox; closed bars: cells maintained in the presence of Dox (1 μg/mL). Results shown are the mean ± SEM of three independent experiments performed. ***; ** relative to absence of Dox, Student’s t-test.
