Oxidative Medicine and Cellular Longevity / 2011 / Article / Fig 3

Research Article

Role of Reactive Oxygen Species in the Neural and Hormonal Regulation of the PNMT Gene in PC12 Cells

Figure 3

Effect of ROS on the hormonal regulation of the PNMT gene. PC12 cells were treated with 200 μM CoCl2, 1  M dexamethasone or the combination of CoCl2 and dexamethasone for 24 h at 37°C. (a) Luciferase activity from cells transfected with the wild-type pGL3RP893 PNMT promoter luciferase reporter gene construct. (b) Representative image ( ) of PNMT (intron-retaining and intronless splice variants) mRNA levels from semiquantitative RT-PCR. GAPDH mRNA was used as a housekeeping control. (c) PNMT intron-retaining mRNA and (d) intronless mRNA. Data is normalized to GAPDH control. Data presented as relative fold change ± SEM from a minimum of three independent experiments. Significance defined as */+ ; ++/## ; ***/+++/### .
(a) Transfection
(b) Representative gel image
(c) PNMT intron-retaining mRNA
(d) PNMT intronless mRNA

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