Role of Reactive Oxygen Species in the Neural and Hormonal Regulation of the PNMT Gene in PC12 Cells
Figure 3
Effect of ROS on the hormonal regulation of the PNMT gene. PC12 cells were treated with 200 μM CoCl2, 1 M dexamethasone or the combination of CoCl2 and dexamethasone for 24 h at 37°C. (a) Luciferase activity from cells transfected with the wild-type pGL3RP893 PNMT promoter luciferase reporter gene construct. (b) Representative image () of PNMT (intron-retaining and intronless splice variants) mRNA levels from semiquantitative RT-PCR. GAPDH mRNA was used as a housekeeping control. (c) PNMT intron-retaining mRNA and (d) intronless mRNA. Data is normalized to GAPDH control. Data presented as relative fold change ± SEM from a minimum of three independent experiments. Significance defined as */+; ++/##; ***/+++/###.
