Role of Oxidative Stress in Transformation Induced by Metal
Mixture
Table 1
Transforming potential through initiation phase.
Initiation phase
Day
Treatmenta
Viability (%)b
Dish with foci/dish scoredc
No. of transformed foci/dish
TPd
1
Control
100 ± 5.5
4/6
1.8 ± 0.05
1 ± 0.27
MNNG + TPA
96 ± 4.8
6/6
62.25 ± 9.9***
35.37 ± 5.6***
Metal mixture + TPA
100 ± 1.7
6/6
33.75 ± 11.6***
18.41 ± 6.3***
MNNG + metal mixture
96 ± 4.8
6/6
23 ± 7.7***
2.61 ± 0.88
Metal mixture + metal mixture
100 ± 1.7
6/6
29.5 ± 5.7***
3.22 ± 0.62
4
Control
100 ± 0.0
4/6
1.8 ± 0.05
1 ± 0.27
MNNG + TPA
89 ± 5.8
6/6
62.25 ± 9.9***
22.7 ± 3.6***
Metal mixture + TPA
89 ± 9.1
6/6
33.75 ± 11.6***
12.4 ± 4.2***
MNNG + metal mixture
89 ± 5.8
6/6
23 ± 7.7***
3.2 ± 1.1
Metal mixture + metal mixture
89 ± 9.1
6/6
29.5 ± 5.7***
1.9 ± 0.4
7
Control
100 ± 0.25
4/6
1.8 ± 0.05
1 ± 0.27
MNNG + TPA
83 ± 2.8**
6/6
62.25 ± 9.9***
83.5 ± 13.4***
Metal mixture + TPA
93 ± 0.12**
6/6
33.75 ± 11.6***
66.5 ± 22.8***
MNNG + metal mixture
83 ± 2.8**
6/6
23 ± 7.7***
6.2 ± 2.1*
Metal mixture + metal mixture
93 ± 0.12**
6/6
29.5 ± 5.7***
9.4 ± 1.8*
a Treatments were added at concentrations of MNNG 0.5 g/mL, TPA 0.1 g/mL, and metal mixture (As 2 M, Cd 2 M, and Pb 5 M); bpercentage with respect to controls; c two experiments with three biological replicates; dtransforming potential (TP) calculated as number of transformed foci type III per dish/surviving cells at corresponding sampling day. Statistical analysis, Student’s t-test value *< 0.05, **< 0.01, and ***< 0.001.
