Antioxidant and Astroprotective Effects of a Pulicaria incisa Infusion

Figure 4

Pi infusion attenuates H₂O₂-induced ROS production in astrocytes. Astrocytes were preloaded with the redox-sensitive DCF-DA for 30 min and washed with PBS. Preloaded astrocytes were then pre-incubated with various concentrations of Pi infusion for 2 h. H₂O₂ (175 μM) was added to the culture and the fluorescence intensity representing ROS production was measured. (a) The fluorescence levels of cells that had been preincubated with 100 μg/mL Pi extract were measured at the indicated time points. ^∗𝑃<0.05,^∗∗𝑃<0.01,^∗∗∗𝑃<0.001 Compared to cells treated with H₂O₂ only. (b) The fluorescence levels of cells that had been pre-incubated with various concentrations of Pi extract were measured after 3 h. Each point represents the mean ± SEM of two experiments (𝑛=8). ^∗𝑃<0.05,^∗∗𝑃<0.01,^∗∗∗𝑃<0.001.

(a)

(b)