Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures
Figure 2
Metabolic activity. The bar chart shows metabolic activity of the cells as determined by resazurin conversion assay at different temperatures and after treatment with or without glyoxal and/or wIRA. Squared brackets indicate statistically significant differences () between temperatures. Cells were able to maintain adequate metabolic activity at temperatures up to 42°C, but their metabolic activity declined significantly at 45°C. The micrographs show cells that were stained with JC-1 for visualising the mitochondrial membrane potential. Red fluorescence, as seen in cells cultured at 37°C, indicates a highly negative mitochondrial membrane potential, while green fluorescence, as seen in cells cultured at 45°C, indicates that the mitochondrial membranes were depolarised. No differences between the treatment groups (with or without glyoxal and/or wIRA) could be observed. Scale bar: 100 μm.
