Effects of Temperature and Water-Filtered Infrared-A Alone or in Combination on Healthy and Glyoxal-Stressed Fibroblast Cultures
Figure 3
Necrosis and apoptosis. Cells were treated with or without glyoxal and/or wIRA at indicated temperatures and were subdued to vital staining with propidium iodide (a), annexin V (b), and YO-PRO-1 (c), as well as staining of fixed cells with propidium iodide for cell cycle analysis (d). Squared brackets indicate statistically significant differences () between temperatures. (a) The percentage of necrotic cells (propidium iodide positive) was significantly higher at 45°C than at the other temperatures but was not affected by glyoxal and/or wIRA treatment. (b) The percentage of apoptotic (annexin V positive) cells was also significantly higher at 45°C than at the other temperatures. (c) The number of apoptotic cells determined by vital staining with YO-PRO-1 was significantly higher at 45°C than at 37°C. (d) Cell cycle analysis revealed that the number of cells with subG1 DNA-content was significantly higher at 45°C than at the other temperatures and was also significantly higher at 37°C than at 42°C.
