313275.fig.004a
(a)
313275.fig.004b
(b)
313275.fig.004c
(c)
313275.fig.004d
(d)
313275.fig.004e
(e)
313275.fig.004f
(f)
Figure 4: TPEN induces activation of NF-κB, p53 and c-Jun transcription factors, caspase-3, and AIF in Jurkat T cells. Jurkat cells (1 × 106 cells/mL) were incubated with TPEN (3 μM) at 37°C for 24 hours. Untreated (a) and TPEN-treated cells were stained with anti-NF-κB-p65 (b), anti-p53 (c), anti-c-Jun (d), anti-caspase-3 (e), and anti-AIF (f) antibodies according to protocol described in according to Material and Methods section Notice that compared to control, NF-κB, p53, c-Jun, caspase-3, and AIF positive nuclei (dark brown color) reflect their nuclear translocation/activation and appear to correlate with the apoptotic nuclear morphology. Notice also that (a) represents the control for each antibody used in ((b)–(f)). Magnification 700x ((a)–(f)).