Bicarbonate Plays a Critical Role in the Generation of Cytotoxicity during SIN-1 Decomposition in Culture Medium
Figure 7
Dependence of SIN-1 cytotoxicity on the total number of initial SIN-1 molecules. (a and b) Treatment volume of medium dependence of SIN-1 cytotoxicity on PC12 cells. Cells in 96-well plates (3000 cells/well) were treated with the indicated concentrations of either freshly prepared SIN-1 in FCS/RPMI (a) or 2 h SIN-1-decomposed FCS/RPMI (b) at either 50 L/well or 100 L/well for 24 h. The cytotoxicity was evaluated by LDH leakage (%). , , and versus respective control. (c and d) Inverse correlation between SIN-1 cytotoxicity and cell density. Cells cultured at different initial densities (1500, 3000, or 6000 cells/well) were treated with the indicated concentrations of freshly prepared SIN-1 in FCS/RPMI (c) or 2 h SIN-1-decomposed FCS/RPMI (d) at 100 L/well for 24 h. , and versus respective 3000 cells/well. (e) Characteristics of NOR-3 cytotoxicity. Cells in 96-well plates (3000 cells/well) were treated for 24 h with the indicated concentrations of freshly prepared NOR-3 in FCS/RPMI or 2 h NOR-3-decomposed FCS/RPMI at either 50 L/well or 100 L/well. Cytotoxicity was measured after 24 h. The values are means ± intra-assay deviations, expressed as SD, from four or five wells in a representative experiment. versus, fresh NOR-3.
