Metformin Rescues the Myocardium from Doxorubicin-Induced Energy Starvation and Mitochondrial Damage in Rats

Figure 6

MET restores DOX-mediated changes in the levels of oxidative stress-related genes. Rats (𝑛=10/treatment type) received normal saline, DOX in a total cumulative dose of 18 mg/kg, MET (50 or 500 mg/kg, p.o., daily), or DOX plus either dose of MET. 24 h after receiving the last dose of DOX, total RNA was isolated from rat cardiac tissue using TRIzol methods and quantified spectrophotometrically at 260 nm. The mRNA levels of GSTα (a), HO-1 (b), CAT (c), and NQO1 (d) were quantified using RT-PCR and normalized to β-actin housekeeping gene. Values represent mean of fold change ± SEM (𝑛=10). ^∗𝑃<0.05 compared with control rats. ⁺𝑃<0.05 compared with DOX-treated rats.

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