Inhibition of Gene Expression of Organic Cation/Carnitine Transporter and Antioxidant Enzymes in Oxazaphosphorines-Induced Acute Cardiomyopathic Rat Models
Figure 5
Effects of cyclophosphamide (CP), ifosfamide (IFO), L carnitine (LC), and their combination on organic cation/carnitine transporter (OCTN2) mRNA (a) and protein (b) expression in rat kidney tissues. Rats were randomly divided into 6 different groups of 10 animals each: control, L carnitine, CP, IFO, CP carnitine supplemented and IFO carnitine supplemented. Carnitine supplementation was induced in rats by daily intraperitoneal injection of L carnitine (200 mg/kg/day) for 10 successive days. CP cardiotoxicity was induced in rats by administration of a single dose of CP (200 mg/kg). IFO cardiotoxicity was induced in rats by administration of IFO (50 mg/kg/day, I.P.) for 5 successive days. CP-carnitine-supplemented rats were given the same doses of L carnitine (200 mg/kg/day) for 5 days before and 5 days after a single dose of CP (200 mg/kg). IFO-carnitine-supplemented rats were given the same doses of L carnitine (200 mg/kg/day) for 5 days before and 5 days concomitant with IFO (50 mg/kg/day, I.P.). At the end of the treatment protocol, total RNA and protein were isolated from kidney tissues, then OCTN2 mRNA and protein expression was measured using RT-PCR and western blot analysis, respectively. Data are presented as mean ± S.E.M. (). *, #, and $ indicate significant change from control, CP and IFO, respectively, at using ANOVA followed by Tukey-Kramer as a post-ANOVA test.
