Insulin Resistance, Ceramide Accumulation, and Endoplasmic Reticulum Stress in Human Chronic Alcohol-Related Liver Disease
Table 3
Effects of chronic ALD on the integrity of the Akt signaling pathway.
Control
Ethanol
value
Protein (Total)
AKT
18599.50 ± 744.87
22377.37 ± 478.47
0.0002
GSK3
2295.38 ± 250.09
1604.090 ± 208.06
0.017
PRAS40
462.13 ± 31.84
438.298 ± 15.13
P70S6K
1204.38 ± 130.96
1258.14 ± 170.01
Protein (phospho)
pS473-Akt
273.47 ± 720.05
252.78 ± 33.53
pS9-GSK3β
612.40 ± 155.72
636.30 ± 46.82
pT246-PRAS40
65.65 ± 5.42
78.07 ± 7.37
pTpS421/424-p70S6K
184.44 ± 23.30
114.32 ± 19.48
Protein (Phospho/Total) * 100
pS473-Akt/Akt
1.43 ± 0.32
1.12 ± 0.14
pS9-GSK3β/GSK3β
28.42 ± 5.12
44.02 ± 8.15
0.038
pT246-PRAS40/PRAS40
14.29 ± 2.26
17.65 ± 1.46
pTpS421/424-p70S6K/p70S6K
13.91 ± 4.14
9.01 ± 0.97
Liver homogenates were used to measure immunoreactivity by multiplex ELISA. Phospho/total signaling protein levels were calculated. Immunoreactivity was normalized to protein concentration and data are expressed as mean ± SEM of fluorescence light units (arbitrary). Intergroup comparisons were made using -tests. Significant values are indicated in the right column.
