NF-B and Matrix-Dependent Regulation of Osteopontin Promoter Activity in Allylamine-Activated Vascular Smooth Muscle Cells
Figure 6
Activity of osteopontin promoter-luciferase constructs in control and allylamine cells seeded on different matrices. Control and allylamine cells were seeded at equivalent densities on collagen I, fibronectin, and laminin, and allowed to recover for 8 hours. Cultures were then transfected with the rat osteopontin promoter (2094 rOPNpr) in a luciferase vector panel (a), and a full length construct in which the NF-κB site was mutated (NF-κBx rOPNpr) panel (b). These mutants are denoted VDREx rOPNPr, AP-1x rOPNpr, NF-κBx rOPNpr, USF1x rOPNpr, USF2x rOPNpr, and USF3x rOPNpr. □: control, ■: allylamine; indicates significantly different from control, + indicates significantly different from 2094 rOPNpr in same cell type on same seeding matrix (). Similar results were seen in multiple experiments.
