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Oxidative Medicine and Cellular Longevity
Volume 2012, Article ID 498914, 14 pages
Research Article

Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids

1Department of Biophysics, “Carol Davila” University of Medicine and Pharmacy, 8 Eroii Sanitari, 050474 Bucharest, Romania
2Istituto Nazionale di Fisica Nucleare, Laboratori Nazionali del Sud, via S. Sofia, 95125 Catania, Italy
3Dipartimento di Fisica e Astronomia, Università di Catania, Viale A. Doria 6, 95125 Catania, Italy
4Sezione di Biochimica e Biologia Molecolare, Dipartimento di Scienze Chimiche, Università di Catania, 6 A. Doria, 95125 Catania, Italy
5“Horia Hulubei” National Institute for Physics and Nuclear Engineering (IFIN-HH), 30 Reactorului, 077125 Bucharest, Romania
6Università degli Studi di Enna “Kore”, Facoltà di Ingegneria, Architettura e delle Scienze Motorie, Cittadella Universitaria, 94100 Enna, Italy

Received 9 March 2012; Accepted 14 May 2012

Academic Editor: Cristina Angeloni

Copyright © 2012 Irina Baran et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

To illustrate the differential effects of various treatments on the cell cycle distribution and the sub-G0/G1 population, we present in Fig. S1 a set of representative DNA-content histograms obtained by flow cytometry after irradiation with 2 Gy of protons of Jurkat cells pre-incubated or not with 50 µM QC for 1 h, or after treatment with 50 µM QC for 1 h, with 250 µM MD for 20 min., or with 500 µM H2O2 for 20 min.

  1. Supplementary Figure