Induction of Mitochondrial Changes Associated with Oxidative Stress on Very Long Chain Fatty Acids (C22:0, C24:0, or C26:0)-Treated Human Neuronal Cells (SK-NB-E)
Figure 6
Analysis of the effects of C22:0, C24:0, and C26:0 on the expression of mitochondrial complex subunits. SK-NB-E cells were cultured for 48 h in the absence (control) or presence of α-cyclodextrin (1 mg/mL) (vehicle), or a VLCFA (C22:0, C24:0, or C26:0) used at 10 μM. The Complex I subunit NDUFB8 (20 kDa), Complex II subunit (30 kDa), Complex III subunit Core 2 (47 kDa), Complex IV subunit II (24 kDa), and Complex V ATP synthase subunit alpha (53 kDa) involved in oxidative phosphorylation obtained from whole cellular extracts were analyzed with specific antibodies (MitoProfile Total OXPHOS Human WB Antibody Cocktail, Abcam/Mitosciences) by Western blotting (a). Quantification was carried out with Image J Software; each complex was normalized versus actin, and for each complex data were expressed as [(normalized value of the assay)/(normalized value of the control)]. Similar values were obtained between control, vehicle, and C22:0-treated cells, and differences were observed especially under treatments with C24:0 and C26:0, mainly for Complex III subunit Core 2 and Complex IV subunit II (b). Data shown are representative of two independent experiments.
