Research Article

Preconditioning and Acute Effects of Flavonoids in Protecting Cardiomyocytes from Oxidative Cell Death

Figure 7

Protection by short- and long-term pretreatment with flavonoids against cell death caused by t-buOOH in H9c2 cells. Long-term pretreatment: cells were kept in DMEM with 2% FBS for 3-day treatment with 25 μM of different flavonoids or 100 μM sodium ascorbate prior to incubating with 400 μM t-buOOH for 24 hr. Live cells were then counted after staining with acridine orange. Short-term pretreatment: cells were kept in DMEM with 2% FBS for 3 days, and treated with 50 μM of different flavonoids or 100 μM sodium ascorbate for 1 h. After the treatment periods, cells were incubated with 400 μM t-buOOH for 24 h, and live cells were counted after staining with acridine orange. Bars show the percent protection upon treatment with flavonoids or ascorbate after incubation with t-buOOH. Asc: ascorbate; Cyan: cyanidin; Del: delphinidin; Cat: catechin; EGCG: epigallocatechin gallate; PC: proanthocyanidins; Quer: quercetin. *Indicates   versus non-treated  t-buOOH group.
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