821936.fig.005a
(a)
821936.fig.005b
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821936.fig.005c
(c)
Figure 5: SFN protected hyperglycemia/hyperlipidemia-induced fibrotic response along with activation of Nrf2 in vitro. HK11 cells were treated with different doses of SFN (0.5 μM–10 μM) for 24 h, and then expression of Nrf2 and HO-1 at protein levels was measured by Western blotting assay (a). HK11 cells were treated with high glucose (HG, 27.5 mM) for 48 h, and, at the last 6 h, palmitate (Pal, 300 μM) was added into the culture medium. Cells were treated with SFN (3 μM) for different times (from 12 h to 48 h), and then expression of CTGF was analyzed by Western blotting assay (b). HK11 cells were treated with HG and SFN for 48 h, and, at the last 6 h, Pal was added into the culture medium as described in panel B. The expression of CTGF, Nrf2, and HO-1 at protein levels was analyzed by Western blotting assay (c). Data are presented as mean ± SD from at least three separate experiments. (*P < 0.05 versus control group; #P < 0.05 versus HG/Pal group).