Prevention of Diabetic Nephropathy by Sulforaphane: Possible Role of Nrf2 Upregulation and Activation

Figure 6

Silencing Nrf2 gene abolished SFN’s prevention of HG/Pal-induced fibrosis. HK11 cells were transfected with Nrf2 siRNA and control siRNA for 48 h and then exposed to SFN (3 μM) and HG (27.5 mM) for additional 48 h. During the second 48 h, Pal (300 μM) were added at the last 6 h. Then the effect of silencing Nrf2 gene with its specific siRNA on SFN-mediated prevention of HG/Pal-induced fibrotic effect along with Nrf2 and HO-1 expression were examined by Western blotting assay. Data are presented as mean ± SD from at least three separate experiments. (*P < 0.05 versus control group; ^#P < 0.05 versus HG/Pal group).