Research Article

The Creation of Cybrids Harboring Mitochondrial Haplogroups in the Taiwanese Population of Ethnic Chinese Background: An Extensive In Vitro Tool for the Study of Mitochondrial Genomic Variations

Table 2

mtDNA copy number of 143B osteosarcoma following EtBr exposure.

Cell typeDays without EtBrmtDNA (copy/cell)

143Bā€”1060.11
08w00.93
8w revertant141009.9
Clone #23500.98
Clone #24500.86
Clone #31500.98
Clone #35500.85
Clone #36500.97
Clone #38500.97
Clone #381100.69

143B: parental osteosarcoma; 8w: 143B following 8 weeks of EtBr exposure; 8w revertant: recovered mtDNA level in the absence of EtBr for 14 days.
Clones 23, 24, 31, 35, 36, and 38 were selected clones through limiting dilution.