Dietary Phenolic Acids Act as Effective Antioxidants in Membrane Models and in Cultured Cells, Exhibiting Proapoptotic Effects in Leukaemia Cells
Figure 6
Effect of phenolic compounds on cell viability/proliferation. Cells were treated with different compounds (5 to 100 μM for HEL cells, (a), (b) and (d); 20 μM for HUVEC, (c)) for 20 hours. (a): Viability was estimated by Trypan Blue exclusion test. (b) and (c): Viability/proliferation was evaluated by MTT assay as described in Section 2. Results are expressed as means ± SD of three independent experiments, each performed in quadruplicate. , significantly different from control cells;, significantly different from control cells;, significantly different from control cells. (d): HEL cells viability, after 20-h treatment with 10, 50, or 100 μM compounds, was determined by the ATPlite 1step luminescence kit as described in Section 2. Results are expressed as means ± SD of three independent experiments, each performed in triplicate. All values are significantly different from control ().
