Group VIB Phospholipase A2 Promotes Proliferation of INS-1 Insulinoma Cells and Attenuates Lipid Peroxidation and Apoptosis Induced by Inflammatory Cytokines and Oxidant Agents
Figure 3
Establishing iPLA 2γ-knockdown INS-1 cell lines. INS-1 cells were transfected with FIV constructs containing inserts that produced either scrambled RNA (control) or siRNA directed against sequences in iPLA2γ mRNA (KD1, KD2). The relative iPLA2γ expression levels in control INS-1 cells and in the iPLA2γ-knockdown (KD) cell lines KD1 and KD2 were assessed by quantitative PCR for mRNA (panel (a)) and by Western blotting analysis for iPLA2γ-immunoreactive protein (panel (b)). In panel (a), mean values ± SEM are displayed (). An asterisk (*) denotes a significant difference () from the value for control INS-1 cells. The immunoblot in panel (b) is representative of four experiments.
(a) Expression of iPLA2γ mRNA by INS-1 cells lines
(b) Immunoblots of expression of iPLA2γ protien by INS-1 cells line
