Rapidly Developing Yeast Microcolonies Differentiate in a Similar Way to Aging Giant Colonies
Figure 4
Physiological differences between Um and Lm cells from 4- and 6-day-old colonies. (a) Oxygen consumption as a measure of respiratory capacity of Um and Lm cells isolated from 4- and 6-day-old microcolonies. Respiration of glucose- and glycerol-grown cells from exponential liquid shaken cultures is shown for comparison. (b) Stress-related features of Um and Lm. Sensitivity to zymolyase is shown as a decrease in optical density of cell suspensions (left). Production of ROS measured as fluorescence of DHE (right). All data represent averages of at least three experiments ±SD. **—t-test value < 0.01; ***—t-test value < 0.001.
