Novel Hematopoietic Target Genes in the NRF2-Mediated Transcriptional Pathway
Figure 6
NRF2-silenced cell lines, erythroid, lymphoid, and KEAP1-silenced protein and mRNA expression of FECH. (a) FECH was significantly decreased in SFN-induced NRF2-silenced mRNA from airway and hepatic cell lines. (b) K562 cells did not respond to chemical treatment at mRNA (10 μM SFN, 24 hours) or protein level (5 hours with 10 μM SFN), unlike the SFN-mediated increase of FECH in lymphoid GM12878 cells. (c) K562 KEAP1KD FECH mRNA was unchanged compared to control. Gene expression values were normalized to BACT or 18S. Bars represent an average of 3–6 independent experiments ± SEM; , . (d) FECH protein did not increase in K562 KEAP1-silenced cells. Protein markers confirmed FECH monomers at ~40 kDA, and the membrane was reprobed with β-actin as a loading control.
