Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity
Effect of HHC and HHDC on PMA stimulated extracellular chemiluminescence of human neutrophils. Human isolated neutrophils (/sample) were stimulated with PMA (0.05 μmol/L) at 37°C in the presence of HHC or HHDC (0.01–100 μmol/L). Extracellular ROS production was measured in the presence of HRP by isoluminol-enhanced chemiluminescence over 1800 s. The values were calculated as percentage of stimulated (PMA) control, on the basis of integrated values of chemiluminescence over 1800 s. Control value given in RLU × seconds was . Mean ± SEM, . ** as compared with the control (PMA) in the absence of the substances tested.