Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 8

Research Article

Study of Possible Mechanisms Involved in the Inhibitory Effects of Coumarin Derivatives on Neutrophil Activity

Figure 8

Phosphorylation of the NADPH oxidase component p40phox in PMA stimulated human neutrophils treated with 10 and 100 μmol/L HHC or HHDC. Human isolated neutrophils (  cells) were incubated at 37°C with HHC or HHDC (10 or 100 μmol/L) for 1 min, prior to addition of PMA (0.15 μmol/L). Cell lysates were prepared, and the protein level p40phox subunit was analyzed by Western blotting and detected by Phospho-p40phox (Thr 154) Antibody. The results are evaluated as optical density of p40phox corrected to optical density of the corresponding β-actin band. Mean ± SEM, (the data are representative of 4 donors performed in 2 separate experiments).