Figure 3: Expression of in SNpc of Wt mice after 6-OHDA injection. (a) representative immunostaining in neurons and astrocytic cells. Immunostaining was carried out using antibodies for (shown in green) together with NeuN (top), OX42 (middle), and GFAP (bottom). Microglia, astrocyte, and neuronal markers are shown in red. In SNpc, -positive cells are basically neurons and astrocytes; however, no -positive cells are OX42-positive cells, thus excluding their microglial origin. In (b), (c), and (d) the graphs represent the percentage of neurons, microglial cells and astrocytic cells expressing , respectively. * and ** (Wt saline versus Wt 6-OHDA).