159567.fig.004a
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159567.fig.004b
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159567.fig.004c
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159567.fig.004d
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Figure 4: Time-lapse fluorescence images at selected time points. (b) Six time-lapse Mito-R, YO-PRO-1 images of normal RBA1 astrocytes. RBA-1 astrocytes become permanent to YO-PRO-1 after treatment with 20 mM H2O2 at 150 min. (d) Five time-lapse Mito-R, YO-PRO-1 images of ρ 0 RBA1 astrocytes. ρ 0 RBA-1 astrocytes become permanent to YO-PRO-1 after treatment with 20 mM H2O2 at 130 min. (a) Phase-contrast images of normal RBA1 astrocytes in six time-lapse series. (c) Phase-contrast images of ρ 0 RBA1 astrocytes in five time-lapse series. Images were taken at rest and after exposure of H2O2 at 0, 10, 30, 60, 70, 130, and 150 min. Bar =10 μm.