Adaptive Redox Response of Mesenchymal Stromal Cells to Stimulation with Lipopolysaccharide Inflammagen: Mechanisms of Remodeling of Tissue Barriers in Sepsis

Figure 12

Confocal immunofluorescence imaging of mitochondrial remodeling in MSCs challenged with LPS. Mitochondrial networks were visualized using projections of TOM20 (red channel), a mitochondrial marker. ((a)-(b)) Control MSCs: mitochondrial network is presented by small-size dots. ((c)-(d)) MSCs challenged with LPS: formation of long-length mitochondrial network occurred due to mitochondrial fusion. Conditions: MSCs were incubated with 500 ng/mL LPS for 3 h. The cells were analyzed 24 h after challenge with LPS. Counterstaining of nuclei was with Hoechst 33342 (blue channel). The confocal images were taken with pinhole setup to obtain 0.5 μm Z-sections.