Adaptive Redox Response of Mesenchymal Stromal Cells to Stimulation with Lipopolysaccharide Inflammagen: Mechanisms of Remodeling of Tissue Barriers in Sepsis

Figure 6

Confocal immunofluorescence imaging of nuclear translocation of NFκB and thioredoxin 1 (TRX1) in MSCs challenged with LPS. ((a)–(c)) Projections of (p65) NFκB (red channel) and TRX1 (green channel) in control MSCs. (d) Overlay of projections of (p65) NFκB, TRX1, and nuclei presented in ((a)–(c)). (e) ROI selected in (d). (f) Histogram of immunofluorescence of (p65) NFκB in nuclei shown in (e). (g) Histogram of immunofluorescence of TRX1 in nuclei shown in (e). ((h)–(j)) Projections of (p65) NFκB (red channel) and TRX1 (green channel) in LPS-challenged MSCs. (k) Overlay of projections of (p65) NFκB, TRX1, and nuclei presented in ((h)–(j)). (l) ROI selected in (k). ((m)-(n)) Histograms of immunofluorescence of (p65) NFκB and TRX1 in nuclei shown in (l). Counterstaining of nuclei was with Hoechst 33342 (blue channel). The confocal images were taken with pinhole setup to obtain 0.5 μm Z-sections. The experimental conditions were the same as indicated in Figure 2.