Research Article

Senescence-Related Changes in Gene Expression of Peripheral Blood Mononuclear Cells from Octo/Nonagenarians Compared to Their Offspring

Table 4

Genes differentially expressed in PBMC of octo/nonagenariansa ( ) versus offspring ( ). Statistical analysis was carried out with t-test. Genes were classified based on functional category.

FunctionGene symbolCorrected valueb valuecFold changedRegulationGene nameEntrez IDe

Cell cycle
BAK1 1.52UpBCL2-antagonist/killer 1NM_001188
CDKN1B 1.43UpCyclin-dependent kinase inhibitor 1B (p27, Kip1)NM_004064
CDK3 1.31UpCyclin-dependent kinase 3 NM_001113324
TRADD 1.48UpTNFRSF1A-associated via death domainNM_003789
CASP2 1.33DownCaspase 2, apoptosis-related cysteine peptidase NM_032982
CDC14A 1.53DownCDC14 cell division cycle 14 homolog A (S. cerevisiae)NM_003672

Immune and related functionCD40LG 1.46DownCD40 ligand (TNF superfamily, member 5, hyper-I gM syndrome)NM_000074
CYSLTR2 1.62DownCysteinyl leukotriene receptor 2NM_020377
IL6ST 1.46DownInterleukin 6 signal transducer (gp130, oncostatin M receptor)NM_002184
TNFSF4 1.93DownTumor necrosis factor (ligand) Superfamily, member 4 (tax-transcriptionally activated glycoprotein 1, 34 kDa)NM_003326

Metabolism
ATP5E 1.30UpATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunitNM_001001977
Oxidative phosphorylation ATP5I 1.26UpATP synthase, H+ transporting, mitochondrial F0 complex, subunit ENM_007100
COX5B 1.24UpCytochrome c oxidase subunit VbNM_001862
COX6A1 1.47UpCytochrome c oxidase subunit VIa polypeptide 1 NM_004373
CYC1 1.19UpCytochrome c-1NM_001916
NDUFA13 1.16UpNADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13NM_015965
NDUFB10 1.16UpNADH dehydrogenase (ubiquinone) 1 beta subcomplex, 10, 22 kDaNM_004548
NDUFS6 1.21UpNADH dehydrogenase (ubiquinone) Fe-S protein 6, 13 kDa (NADH-coenzyme Q reductase)NM_004553
NDUFV1 1.16UpNADH dehydrogenase (ubiquinone) flavoprotein 1, 51 kDaNM_007103
SDHA 1.21UpSuccinate dehydrogenase complex, subunit A, flavoprotein (Fp)NM_004168
SDHB 1.19UpSuccinate dehydrogenase complex, subunit B, iron sulfur [6]NM_003000
UQCR 1.21UpUbiquinol-cytochrome c reductase, 6.4 kDa subunitNM_006830
UQCRFS1 1.39UpUbiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1NM_006003
ATP2A2 1.37DownATPase, Ca++ transporting, cardiac muscle, slow twitch 2NM_170665
ATP2C1 1.22DownATPase, Ca++ transporting, type 2C, member 1NM_014382
COX2 1.19DownCytochrome c oxidase IINC_001807
ISCA1 1.21DownIron-sulfur cluster assembly 1 homolog (S. cerevisiae)NM_030940
Insulin action IGF2R 1.28DownInsulin-like growth factor 2 receptorNM_000876
PIK3R4 1.21Downphosphoinositide-3-kinase, regulatory subunit 4NM_014602
PIP3-E 1.34DownNM_015553
PIP5K3 1.17DownPhosphatidylinositol-3-phosphate/phosphatidylinositol 5-kinase, type IIINM_015040
PRKACB 1.44DownProtein kinase, cAMP-dependent, catalytic, betaNM_182948
PRKCA 1.38DownProtein kinase C, alphaNM_002737
Cholesterol,  lipid and,  lipoprotein maintenance LRPAP1 1.47UpLow density lipoprotein receptor-related protein associated protein 1NM_002337
SCD 1.23DownStearoyl-CoA desaturase (delta-9-desaturase)NM_005063

Response to stress
FOXO4 1.27UpForkhead box O4NM_005938
HMOX2 1.19UpHeme oxygenase (decycling) 2NM_002134
PIN1 1.24UpPeptidylprolyl cis/trans isomerase, NIMA-interacting 1NM_006221
PRDX2 1.15UpPeroxiredoxin 2NM_005809
PRDX5 1.27UpPeroxiredoxin 5NM_012094
HSPA8 1.47DownHeat shock 70 kDa protein 8NM_006597

DNA repair and telomere maintenance
CIB1 1.20UpCalcium and integrin binding 1 (calmyrin)NM_006384
FEN1 1.24UpFlap structure-specific endonuclease 1NM_004111
TERF2IP 1.16UpTelomeric repeat binding factor 2, interacting proteinNM_018975
TINF2 1.31UpTERF1 (TRF1)-interacting nuclear factor 2NM_012461
XRCC6 1.27UpX-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70 kDa)NM_001469
DICER1 1.28DownDicer 1, ribonuclease type IIINM_177438

Translation and transcription
USF1 1.33UpUpstream transcription factor 1NM_007122
KEAP1 1.23UpKelch-like ECH-associated protein 1NM_203500
NRF1 1.19UpNuclear respiratory factor 1NM_005011
SIRT7 1.18UpSirtuin (silent mating type information regulation 2 homolog) 7 (S. cerevisiae)NM_016538

AutophagyBECN1 1.32UpBeclin 1, autophagy relatedNM_003766
VPS18 1.26UpVacuolar protein sorting 18 homolog (S. cerevisiae)NM_020857

The genes shown in this table are ordered according to the biological processes. For a complete list of all 812 genes significantly affected in octo/nonagenarians, see Table S2.
bCorrected value refers to value after multiple testing corrections by Benjamini-Hochberg method.
cP value refers to value before multiple testing corrections by Benjamini-Hochberg method.
dThe fold change refers to the ratio of the expression values of octo/nonagenarians over offspring.
eSource of entrez ID is from National Center for Biotechnology Information (NCBI), http://www.ncbi.nlm.nih.gov/gene/.