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Oxidative Medicine and Cellular Longevity
Volume 2013 (2013), Article ID 194192, 8 pages
http://dx.doi.org/10.1155/2013/194192
Research Article

Is Oxidative Stress in Mice Brain Regions Diminished by 2-[(2,6-Dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile?

1Postgraduate Program in Pharmaceutical Sciences, Federal University of PI, 64.049-550 Teresina, Piauí, Brazil
2Department of Pharmacy, Federal University of Piaui, 64.049-550 Teresina, PI, Brazil
3Federal University of Sergipe, Center for Biological and Health Sciences, Department of Morphology, 49.100-000 São Cristovão, SE, Brazil
4Laboratory of Synthesis and Vectorization of Molecules, State University of Paraiba, 58.020-540 João Pessoa, PB, Brazil
5Department of Pharmaceutical Sciences, Federal University of Pernambuco, 50740-520 Recife, PE, Brazil

Received 16 October 2012; Revised 11 January 2013; Accepted 31 January 2013

Academic Editor: Emilio Luiz Streck

Copyright © 2013 A. C. Fortes et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

2-[(2,6-Dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile, 5TIO1, is a new 2-aminothiophene derivative with promising pharmacological activities. The aim of this study was to evaluate its antioxidant activity in different areas of mice central nervous system. Male Swiss adult mice were intraperitoneally treated with Tween 80 dissolved in 0.9% saline (control group) and 5TIO1 (0.1, 1, and 10 mg kg−1). Brain homogenates—hippocampus, striatum, frontal cortex, and cerebellum—were obtained after 24 h of observation. Superoxide dismutase and catalase activities, lipid peroxidation and nitrite content were measured using spectrophotometrical methods. To clarify the 5TIO1’s mechanism on oxidative stress, western blot analysis of superoxide dismutase and catalase was also performed. 5TIO1 decreased lipid peroxidation and nitrite content in all brain areas and increased the antioxidant enzymatic activities, specially, in cerebellum. The data of Western blot analysis did not demonstrate evidence of the upregulation of these enzymes after the administration of this compound. Our findings strongly support that 5TIO1 can protect the brain against neuronal damages regularly observed during neuropathologies.