Is Oxidative Stress in Mice Brain Regions Diminished by 2-[(2,6-Dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carbonitrile?
Superoxide dismutase and catalase proteins in hippocampus, striatum, frontal cortex, and cerebellum of mice treated with vehicle and 5TIO1. Male mice (25–30 g, 2 month-old) were intraperitoneally treated with 0.25 mL of 0.05% Tween 80 dissolved in 0.9% saline (vehicle) (, control group), and 5TIO1 groups with 2-[(2,6-dichlorobenzylidene)amino]-5,6-dihydro-4H-cyclopenta [b]thiophene-3-carbonitrile emulsified in vehicle (0.1, 1, and 10 mg/kg, , 5TIO1 groups). Animals were observed for 24 h and then killed. The protein amount per lane was 10 Ag. Legends: (I) control group; (II) 5TIO1 0.1 mg/kg group; (III) 5TIO1 1 mg/kg group; (IV) 5TIO1 10 mg/kg group. Data are expressed as means ± SD, converted to percentage of control (set at 100%) experiments; Statistical analysis was by one-way ANOVA and -Student-Neuman-Keuls test as post hoc test. There were no significant differences in protein expression of catalase after treatments, although there was a significant difference ().