Carbon Monoxide Attenuates Dextran Sulfate Sodium-Induced Colitis via Inhibition of GSK-3β Signaling
Figure 4
CO downregulates TNF-α and iNOS expression via inhibition of GSK-3β signaling. (a) to (c) U937 cells were pretreated with CORM2 (100 μM) and LiCl (20 mM) for 30 min followed by stimulation with LPS (1 μg/mL) for 24 h. (a) TNFα and iNOS mRNA expression was measured by RT-PCR, (b) iNOS protein expression was measured by western blotting, and (c) supernatant was collected and TNF-α protein level was measured by ELISA. (d) to (e) MLN cells were pre-incubated with CORM2 (100 μM) and LiCl (20 mM) for 30 min and then stimulated with LPS (1 μg/mL) for 24 h. (d) iNOS and TNF-α mRNA expression was measured by RT-PCR, and (e) Supernatant was used to measure TNF-α protein level by ELISA. The representative band or blot is shown. Data represents mean ± SEM, *, **, ***.
