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Oxidative Medicine and Cellular Longevity
Volume 2013 (2013), Article ID 234179, 10 pages
http://dx.doi.org/10.1155/2013/234179
Research Article

Comparative Effects of Bone Marrow Mesenchymal Stem Cells on Lipopolysaccharide-Induced Microglial Activation

1Department and Institute of Pharmacology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan
2Neural Regeneration Laboratory, Department of Neurosurgery, Neurological Institute, Taipei Veterans General Hospital, Taipei 11221, Taiwan
3Center for Neural Regeneration, Neurological Institute, Taipei Veterans General Hospital, Taipei 11221, Taiwan
4Department of Anatomy and Cell Biology, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan
5Department of Education and Research, Taipei City Hospital, Taipei 11221, Taiwan
6Faculty of Medicine, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan
7Institute of Brain Science, School of Medicine, National Yang-Ming University, Taipei 11221, Taiwan

Received 6 December 2012; Accepted 14 February 2013

Academic Editor: Anantharaman Muthuswamy

Copyright © 2013 Fan-Wei Tseng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

After injury to the CNS, microglia are rapidly activated and concentrated and trigger inflammatory reaction at the sites of injury. Bone marrow mesenchymal stem cells (BMMSC) represent attractive cell sources for treating CNS injury. Although anti-inflammatory and paracrine effects of grafted BMMSC have been shown, direct modulation of BMMSC on microglia in situ remains unclear. The present work employs in vitro transwell assay to characterize the effects of BMMSC on LPS-stimulated microglia. BMMSC are cultivated in serum and serum-free (sf) conditions, namely, BMMSC and BMMSC-sf. Both cultures express major surface markers specific for mesenchymal stem cells. However, the BMMSC-sf exhibit sphere-like structure with reduced expression of two adherent cell markers, CD29 and CD90. Compared to BMMSC-sf, BMMSC are fibroblast like and have faster differentiation potential into neural-like cells. Furthermore, BMMSC release significant levels of TIMP-1 and VEGF, regardless of being alone or in coculture. The downregulated MMP-9 mRNA may be caused by TIMP-1 secretion from BMMSC. Our cell culture system provides a powerful tool for investigating the molecular and cellular changes in microglia-BMMSC cocultures.