Research Article

Hemeoxygenase-1 Mediates an Adaptive Response to Spermidine-Induced Cell Death in Human Endothelial Cells

Figure 1

Induction of HO-1 by SPD in HUVECs. After treatment of HUVECs with various concentrations of SPD (a) at various time intervals (b), cell lysates were prepared, and 20 μg samples of proteins were subjected to Western blotting using the anti-HO-1 antibody, and anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody as a loading control. Representative data from three independent experiments are shown. After treatment of HUVECs with various concentrations of SPD (c) at various time intervals (d), cell lysates were prepared. The total RNA was extracted and analyzed by RT-PCR. The amplified RT-PCR product was visualized on 1% agarose gel. Representative data from three independent experiments are shown.
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(b)
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(c)
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(d)