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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 303181, 9 pages
Research Article

Age-Dependent Accumulation of 8-Oxoguanine in the DNA and RNA in Various Rat Tissues

1Graduate School, Wenzhou Medical College, University Town, Wenzhou, Zhejiang 325035, China
2The Key Laboratory of Geriatrics, Beijing Hospital and Beijing Institute of Geriatrics, Ministry of Health, No. 1 Dahua Road, Dongdan, Beijing 100730, China
3Graduate School, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 9 Santiao, Dongdan, Dongcheng District, Beijing 100730, China
4Department of Pharmacology, Wenzhou Medical College, University Town, Wenzhou, Zhejiang 325035, China
5Frontier Research Center, Fukuoka Dental College, Fukuoka 814-0193, Japan

Received 4 February 2013; Accepted 20 March 2013

Academic Editor: Gabriele Saretzki

Copyright © 2013 Ben Nie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The relationship between the oxidative damage of nucleic acids and aging of animals was investigated by analyzing the nucleic acids derived from various tissue specimens of naturally aged Sprague-Dawley (SD) rats. For this purpose, we established an accurate and sensitive isotope-diluted LC-MS/MS method to determine the levels of 8-oxo-7,8-dihydro- -deoxyguanosine (8-oxo-dGsn) in DNA and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn) in RNA. An age-dependent increase in oxidative DNA and RNA damage was observed in the various organs examined, including the brain, liver, kidneys, and testes. Similar increases in the 8-oxo-dGsn and 8-oxo-Gsn contents were observed in three parts of the brain, the hippocampus, cerebral cortex, and cerebellum, among which, the values for the hippocampus were always the highest. When the oxidized guanosine metabolites were quantified with urine, a similar age-dependent increase was observed for both 8-oxo-dGsn and 8-oxo-Gsn. However, unlike the results of nucleic acid samples derived from the tissues, the amount of 8-oxo-Gsn was significantly higher compared to that of 8-oxo-dGsn, probably reflecting the fact that RNA degradation occurs more frequently than DNA degradation. Our finding indicates that the amount of urinary 8-oxo-Gsn could be considered as a biomarker for the sensitive measurement of oxidative stress and aging.