Mitochondrial-Targeted Two-Photon Fluorescent Probes for Zinc Ions, H_𝟐O_𝟐, and Thiols in Living Tissues

Figure 1

(a) TPM and (b) OPM images of HeLa cells colabeled with (a) SZn2-Mito (1 μM) and (b) Mitotracker Red FM (1 μM). (c) Colocalized image. The wavelengths for one- and two-photon excitations were 514 and 760 nm, respectively, and the emission was collected at 450–550 (SZn-Mito) and 600–700 nm (Mitotracker Red FM), respectively. Scale bar, 20 μm. (d–f) TPM images of 1 μM SZn2-Mito-labeled HeLa cells, before (d) and after (e) addition of 150 μM DTDP to the imaging solution. (f) After addition of 10 μM CCCP to (e). (g) The relative TPEF intensity of SZn2-Mito-labeled HeLa cells as a function of time. The TPEF intensities were collected at 450–600 nm upon excitation at 750 nm with fs pulse. Scale bar, 10 μm. Cells shown are representative images from replicate experiments (𝑛=5) [10].