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Oxidative Medicine and Cellular Longevity
Volume 2013, Article ID 325406, 11 pages
http://dx.doi.org/10.1155/2013/325406
Research Article

Ameliorating Effect of Various Fractions of Rumex hastatus Roots against Hepato- and Testicular Toxicity Caused by CCl4

1Botanical Sciences Division, Pakistan Museum of Natural History, Garden Avenue, Shakarparian, Islamabad, Pakistan
2Department of Biochemistry, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan
3Department of Biotechnology, Faculty of Biological Sciences, University of Science and Technology, Bannu, Khyber Pakhtunkhwa 28100, Pakistan

Received 14 February 2013; Revised 4 April 2013; Accepted 4 April 2013

Academic Editor: Kota V. Ramana

Copyright © 2013 Sumaira Sahreen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Effect of methanolic extract of Rumex hastatus roots (MRR) and its derived fractions, n-hexane (HRR), ethyl acetate (ERR), chloroform (CRR), butanol (BRR), and aqueous extract (ARR), was studied against carbon tetrachloride (CCl4) induced hepato and testicular toxicity in rats. Intraperitoneal dose of 20 percent CCl4 (0.5 ml/kg bw) was administered twice a week for eight weeks to a group of rats. Other groups were given CCl4 and various fractions of R. hastatus roots (200 mg/kg bw). CCl4 treatment depleted glutathione contents and activities of antioxidant enzymes while increased the concentration of lipid peroxides (TBARS) along with corresponding DNA injuries and histopathological damages. Supplementation with various fractions of R. hastatus roots (200 mg/kg body weight) attenuated the toxicity of CCl4 in liver and testis tissues through improvement in the serological, enzymatic, and histological parameters towards the normal. Posttreatment of R. hastatus roots (200 mg/kg body weight) also reversed the alteration in reproductive hormonal secretions and DNA damages in CCl4 treated rats. The results clearly demonstrated that R. hastatus treatment augments the antioxidants defense mechanism and provides the evidence that it may have a therapeutic role in free radical mediated diseases.