Research Article

Steviol Glycosides Modulate Glucose Transport in Different Cell Types

Figure 5

Effect of steviol glycosides on ROS levels in SH-SY5Y and HL-60 cells. SH-SY5Y (a) and HL-60 (b) cells were treated for 1 hour with different compounds (5 mg/mL); then basal ROS levels were measured by means of H2DCFDA assay as described in Section 2. Results are expressed as means ± SD of four independent experiments ( ). Statistical analysis was performed by Bonferroni multiple comparison test following one-way ANOVA. Significant differences were not revealed. SH-SY5Y (c) and HL-60 (d) cells were preincubated for 1 hour with different compounds (5 mg/mL) and then exposed to oxidative stress generated by 100 μM H2O2 for 30 min. ROS levels were measured by means of H2DCFDA assay as described in Section 2. Results are expressed as means ± SD of four independent experiments ( ). Statistical analysis was performed by Bonferroni multiple comparison test following one-way ANOVA. Significant differences were not revealed among H2O2 treated cells.
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