Steviol Glycosides Modulate Glucose Transport in Different Cell Types
Figure 8
Effects of Stevia extracts on PI3K/Akt pathway. SH-SY5Y and HL-60 cells, treated with insulin or with steviol glycosides, were lysed with CelLytic M as described in Section 2. Cell lysates were electrophoresed and immunoblotted with the indicated antibodies, as described in Section 2. β-Actin detection was used as a control. Immunoblots representative of three independent experiments are reported for SH-SY5Y (a) and HL-60 (b) cell lines; densitometric analysis of PI3K phosphorylation status is expressed as phospho-PI3K/total PI3K ratio (c) and densitometric analysis of Akt phosphorylation status is expressed as phospho-Akt/total Akt ratio (d). *, significantly different from control cells.
