Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 7

Research Article

Tanshinol Attenuates the Deleterious Effects of Oxidative Stress on Osteoblastic Differentiation via Wnt/FoxO3a Signaling

Figure 7

Effects of Tanshinol on Tcf- and FoxO3a-mediated transcription activity in C2C12 cells and MC3T3-E1 cells treated with FoxO3a siRNA, or with overexpression of β-Catenin or Tcf4 under oxidative stress. ((a), (c), and (e)) C2C12 cells or MC3T3-E1 cells were cotransfected with the FoxO3a-luc reporter plasmid in combination with FoxO3a siRNA, or pcDNA3-β-catenin, or pcDNA3-Tcf4, or corresponding control using Attractene transfection reagent. Other procedures were carried out as described in Figure 5(b). ((b), (d), and (f)) Cells were cotransfected with Tcf-luc reporter plasmid and other treatment described in (a), (c), and (e). Others procedure were made as described in Figure 5(b). Bars indicate mean ± SME of triplicate determinations. versus vehicle control and versus H2O2 treatment; versus corresponding empty vector or scrambled control.

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