Nordihydroguaiaretic Acid Attenuates the Oxidative Stress-Induced Decrease of CD33 Expression in Human Monocytes

Figure 2

Protective effect of NDGA on the cytotoxicity-induced by ((a), (c), and (e)) IAA and ((b), (d), and (f)) H₂O₂ in human cells. ((a), (b)) MN and ((c), (d), (e), (f)) THP-1 cells were pretreated in absence or presence of 20 μM NDGA by 12 h. Cell cultures were exposed to IAA or H₂O₂ for 2 h and refreshed after removal of the toxic compounds. MTT reduction or trypan blue exclusion was determined 24 h after the onset of IAA or H₂O₂ exposure. The number of viable cells is expressed as ((a), (b), (c), (d)) index of MTT reduction or ((e), (f)) percentage of trypan blue negative cells. Data are expressed as mean ± SEM; 𝑛=5. ⁺𝑃<0.05 versus control (without IAA or H₂O₂) and ^∗𝑃<0.05 versus control (without NDGA).

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