Nordihydroguaiaretic Acid Attenuates the Oxidative Stress-Induced Decrease of CD33 Expression in Human Monocytes
Figure 5
Effect of IAA or H2O2 on GSH + GSSG and GSH levels in THP-1 cells. Cells were treated 24 h with (a) IAA or (b) H2O2. After this time these compounds were withdrawn and fresh medium was added. GSSG and GSH levels were measured 24 h after the addition of toxic compounds. In addition the effect of NDGA on GSSG + GSH and GSH levels in (c) IAA or (d) H2O2 treated cells was also studied. Cells were treated with vehicle or 20 μM NDGA by 12 h; after this the cells were exposed to 5 μM IAA or 1 mM H2O2 for 24 h. GSSG and GSH levels were measured 24 h after the addition of toxic compounds. GSH levels were quantified by spectrophotometric/microplate reader assay. Data are expressed as mean ± SEM; . versus control (without IAA or H2O2) and versus the respective toxic compound.
