Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
Figure 2
Propofol increased the viability and reduced the apoptosis of the H/R induced injury in HUVECs. (a) The normal HUVECs were treated with different concentrations (0–150 μmol/L) of propofol for 4 h. (b) The cells were postconditioned with increasing concentrations of propofol (0–150 μmol/L) after 12 h of hypoxia and 4 h of reperfusion. Cell viability was determined by CCK-8 assay, as previously described. Values are represented as the percentage of viable cells; vehicle-treated cells were considered as 100% viable. The data represented are mean percentage of viable cells + SD of three independent experiments. *, compared with control group, ▼, compared with the H/R group. (c) Detection of apoptosis with Annexin V-FITC and propidium iodide staining. Every group of cells with Annexin V and propidium iodide staining was measured by flow cytometry. Histogram representing the percentage of early apoptotic cells and late apoptotic cells. The data represent the mean + SD of three independent experiments. *, compared with the control group, and ▼ with H/R group. (d) Expression of apoptosis-related proteins in normal group, H/R injury group, and propofol posthypoxia treatment groups. Data are representative WB from 3 independent experiments ().
