Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
Table 2
Primers used in qRT-PCR.
Gene
Annealing temperature °C
Number of gene primer (5′3′)
Accession number
U6
60
F: 5′CTCGCTTCGGCAGCACATATACT3′
NR_004394.1
R: 5′CGAATTTGCGTGTCATCCTTGCG3′
hsa-miR-30b
60
F: 5′CGCTGTAAACATCCTACACTCA3′
MIMAT0000420
R: 5′GCAGGGTCCGAGGTATTC3′
Hsa-miR-15b-5P
60
F: 5′ATGGTTCGTGGGTAGCAGCACATCATGGTTTACA3′
MIMAT0000417
R: 5′GCAGGGTCCGAGGTATTC3′
Has-miR-196a-5p
60
F: 5′ATGGTTCGTGGGTAGGTAGTTTCATGTTGTTGG3′
MIMAT0000226
R: 5′GCAGGGTCCGAGGTATTC3′
Hsa-miR-374b-5P
60
F: 5′CGTGGGATATAATACAACCTGCTAAGTG3′
MIMAT0004955
R: 5′CTCAACTGGTGTCGTGGA3′
Hsa-miR-20b-5P
60
F: 5′ATGGTTCGTGGGCAAAGTGCTCATAGTGCAGGTAG3′
MIMAT0001413
R: 5′CTCAACTGGTGTCGTGGA3′
Hsa-let-7e
60
F: 5′CGCTGAGGTAGGAGGTTGTA3′
MIMAT0000066
R: 5′GCAGGGTCCGAGGTATTC3′
F: forward primer; R: reverse primer. miRNA number and sequence of a specific miRNA can be obtained from miRBase sequence.
